Phosphate Buffered Saline (PBS) Solution
From StemNode Lit Reviews
Contents
Authors:
This method is also called: PBS
This method is adapted from:
Discussion
Useful for keeping cells under osmotic equilibrium.
Materials and Equipment
- 1L Volumetric Flask
- Magnetic stir plate and stir bar
Reagents
| Reagent | Concentration | Units | CAS Number |
|---|---|---|---|
| Sodium Chloride | 100 | % | 7647-14-5 |
| Sodium Phosphate Dibasic Anhydrous | 100 | % | 7558-79-4 |
| Sodium Phosphate Monobasic Monohydrous | 100 | % | 7558-80-7 |
Procedure
To 1L volumetric flask add 8.5g Sodium Chloride (NaCl), 1.2 g Sodium Phosphate Dibasic Anydrous (Na2HPO4), 0.9g Sodium Phosphate Monobasic Monohydrous (NaH2PO4*H2O). Top with DI Water. Place on stir plate until fully dissolved.
Common Mistakes
| Mistake | Evidence of the Mistake | Cause | Effect on Results |
|---|---|---|---|
