View source for Phosphate Buffered Saline (PBS) Solution


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==== '''This method is also called:''' PBS ====

==== '''This method is adapted from: <!-- Citation -->''' ====

== Discussion ==<!-- Discuss the purpose, the suitability of the method for certain situations, and the fundamentals science behind the method.-->
Useful for keeping cells under osmotic equilibrium. 

== Materials and Equipment ==
* 1L Volumetric Flask
* Magnetic stir plate and stir bar<!-- Any tubes, pipette tips ect. -->

==Reagents==<!-- Test kits go here if used-->
{| class="wikitable sortable"
|-
! Reagent
! Concentration
! Units
! CAS Number 
|-
| Sodium Chloride<!-- Common Name-->
| 100<!-- A value-->
| <!-- By weight or volume? g/L, Mol, ect..-->%
| <!-- Universal Identifier, regardless of manufacturer -->7647-14-5
|-
|Sodium Phosphate Dibasic Anhydrous
|100
|%
|7558-79-4
|-
|Sodium Phosphate Monobasic Monohydrous 
|100
|%
|7558-80-7
|}

==Procedure==

To 1L volumetric flask add 8.5g Sodium Chloride (NaCl), 1.2 g Sodium Phosphate Dibasic Anydrous (Na2HPO4), 0.9g Sodium Phosphate Monobasic Monohydrous (NaH2PO4*H2O). Top with DI Water. Place on stir plate until fully dissolved. 

==Common Mistakes==

{| class="wikitable sortable"
|-
! Mistake
! Evidence of the Mistake 
!Cause
! Effect on Results
|-
| 
| <!-- (How can you tell if the mistake has been made?)(How can you tell if the mistake has been made?) -->
|<!-- What is fundametnally happening
 -->
| <!-- (Higher, lower, flatline?) -->
|}

==Precision and Interference==