Difference between revisions of "Phosphate Buffered Saline (PBS) Solution"
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===={{Template:CiteAuthors}} ==== | ===={{Template:CiteAuthors}} ==== | ||
| − | ==== '''This method is also called:''' ==== | + | ==== '''This method is also called:''' PBS ==== |
==== '''This method is adapted from: <!-- Citation -->''' ==== | ==== '''This method is adapted from: <!-- Citation -->''' ==== | ||
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Useful for keeping cells under osmotic equilibrium. | Useful for keeping cells under osmotic equilibrium. | ||
| − | == Materials and Equipment ==<!-- Any tubes, pipette tips ect. --> | + | == Materials and Equipment == |
| + | * 1L Volumetric Flask | ||
| + | * Magnetic stir plate and stir bar<!-- Any tubes, pipette tips ect. --> | ||
==Reagents==<!-- Test kits go here if used--> | ==Reagents==<!-- Test kits go here if used--> | ||
| Line 23: | Line 25: | ||
! CAS Number | ! CAS Number | ||
|- | |- | ||
| − | | <!-- Common Name--> | + | | Sodium Chloride<!-- Common Name--> |
| − | | <!-- A value--> | + | | 100<!-- A value--> |
| − | | <!-- By weight or volume? g/L, Mol, ect..--> | + | | <!-- By weight or volume? g/L, Mol, ect..-->% |
| − | | <!-- Universal Identifier, regardless of manufacturer --> | + | | <!-- Universal Identifier, regardless of manufacturer -->7647-14-5 |
| + | |- | ||
| + | |Sodium Phosphate Dibasid Anhydrous | ||
| + | |100 | ||
| + | |% | ||
| + | |7558-79-4 | ||
| + | |- | ||
| + | |Sodium Phosphate Monobasic Monohydrous | ||
| + | |100 | ||
| + | |% | ||
| + | |7558-80-7 | ||
|} | |} | ||
==Procedure== | ==Procedure== | ||
| − | To 1L volumetric flask add 8.5g Sodium Chloride (NaCl), 1.2 g Sodium Phosphate Dibasic Anydrous (Na2HPO4 | + | To 1L volumetric flask add 8.5g Sodium Chloride (NaCl), 1.2 g Sodium Phosphate Dibasic Anydrous (Na2HPO4), 0.9g Sodium Phosphate Monobasic Monohydrous (NaH2PO4*H2O). Top with DI Water. Place on stir plate until fully dissolved. |
==Common Mistakes== | ==Common Mistakes== | ||
Revision as of 04:00, 1 March 2019
Contents
Authors:
This method is also called: PBS
This method is adapted from:
Discussion
Useful for keeping cells under osmotic equilibrium.
Materials and Equipment
- 1L Volumetric Flask
- Magnetic stir plate and stir bar
Reagents
| Reagent | Concentration | Units | CAS Number |
|---|---|---|---|
| Sodium Chloride | 100 | % | 7647-14-5 |
| Sodium Phosphate Dibasid Anhydrous | 100 | % | 7558-79-4 |
| Sodium Phosphate Monobasic Monohydrous | 100 | % | 7558-80-7 |
Procedure
To 1L volumetric flask add 8.5g Sodium Chloride (NaCl), 1.2 g Sodium Phosphate Dibasic Anydrous (Na2HPO4), 0.9g Sodium Phosphate Monobasic Monohydrous (NaH2PO4*H2O). Top with DI Water. Place on stir plate until fully dissolved.
Common Mistakes
| Mistake | Evidence of the Mistake | Cause | Effect on Results |
|---|---|---|---|
