Phosphate Buffered Saline (PBS) Solution

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Authors:

This method is also called:

This method is adapted from:

Discussion

Useful for keeping cells under osmotic equilibrium.

Materials and Equipment

Reagents

Reagent Concentration Units CAS Number

Procedure

To 1L volumetric flask add 8.5g Sodium Chloride (NaCl), 1.2 g Sodium Phosphate Dibasic Anydrous (Na2HPO4, 333-D), 0.9g Sodium Phosphate Monobasic Monohydrous (NaH2PO4*H2O). Top with DI Water.

Common Mistakes

Mistake Evidence of the Mistake Cause Effect on Results

Precision and Interference